rabbit polyclonal anti rat pd  (Proteintech)

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Early changes of NLRP3 inflammasome activation after hypoxic-ischemic brain injury in neonatal rats

doi:

Figure Lengend Snippet: Primer sequences used for quantitative polymerase chain reaction

Article Snippet: After blocking for 1 h with 5% skim milk powder, the membrane was incubated overnight at 4°C with mouse anti-rat caspase-1 antibody (1:500), rabbit anti-rat NLRP3 polyclonal antibody (1:1000; ab214185; Abcam), or rabbit anti-rat IL-1β polyclonal antibody (1:1000; ab205924, Abcam).

Techniques: Sequencing

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Early changes of NLRP3 inflammasome activation after hypoxic-ischemic brain injury in neonatal rats

doi:

Figure Lengend Snippet: Expression of NLRP3, caspase-1, and IL-1β proteins in brain tissue evaluated by immunohistochemistry. A. Representative images showing sections of brain tissue from the HI group stained using immunohistochemistry techniques. The expression levels of NLRP3, caspase-1, and IL-1β proteins were determined at 0 h, 4 h, 8 h, 12 h, and 24 h after surgery/hypoxia. B. Averaged semi-quantitative data comparing the expression levels of NLRP3, caspase-1, and IL-1β proteins between the various time points. **P < 0.01, ***P < 0.001 vs. 0 h.

Article Snippet: After blocking for 1 h with 5% skim milk powder, the membrane was incubated overnight at 4°C with mouse anti-rat caspase-1 antibody (1:500), rabbit anti-rat NLRP3 polyclonal antibody (1:1000; ab214185; Abcam), or rabbit anti-rat IL-1β polyclonal antibody (1:1000; ab205924, Abcam).

Techniques: Expressing, Immunohistochemistry, Staining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Early changes of NLRP3 inflammasome activation after hypoxic-ischemic brain injury in neonatal rats

doi:

Figure Lengend Snippet: Expression of NLRP3, caspase-1, and IL-1β proteins in brain tissue evaluated by western blot. A. Representative immunoblots illustrating the expressions levels of NLRP3, caspase-1, and IL-1β proteins in brain tissue from the HI and sham operation groups at varying times (0 h, 4 h, 8 h, 12 h and 24 h) after surgery/hypoxia. B. Averaged semi-quantitative data comparing the expression levels of NLRP3, caspase-1, and IL-1β proteins between the HI and sham operation groups. *P < 0.05, **P < 0.01, ***P < 0.001 vs. sham operation group.

Article Snippet: After blocking for 1 h with 5% skim milk powder, the membrane was incubated overnight at 4°C with mouse anti-rat caspase-1 antibody (1:500), rabbit anti-rat NLRP3 polyclonal antibody (1:1000; ab214185; Abcam), or rabbit anti-rat IL-1β polyclonal antibody (1:1000; ab205924, Abcam).

Techniques: Expressing, Western Blot

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Early changes of NLRP3 inflammasome activation after hypoxic-ischemic brain injury in neonatal rats

doi:

Figure Lengend Snippet: Expression of NLRP3, caspase-1, and IL-1β mRNA in brain tissue evaluated by RT-PCR. Averaged semi-quantitative data are shown comparing the expression levels of NLRP3, caspase-1, and IL-1β mRNA in brain tissue between the HI and sham operation groups at varying times (0 h, 4 h, 8 h, 12 h and 24 h) after surgery/hypoxia. *P < 0.05, **P < 0.01, ***P < 0.001 vs. sham operation group.

Article Snippet: After blocking for 1 h with 5% skim milk powder, the membrane was incubated overnight at 4°C with mouse anti-rat caspase-1 antibody (1:500), rabbit anti-rat NLRP3 polyclonal antibody (1:1000; ab214185; Abcam), or rabbit anti-rat IL-1β polyclonal antibody (1:1000; ab205924, Abcam).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction

Journal: Cancer

Article Title: Overall survival and PD-L1 expression in metastasized malignant melanoma.

doi: 10.1002/cncr.25747

Figure Lengend Snippet: Figure 1. Testing available antihuman-PD-L1 antibodies for paraffin immunohistochemistry is shown. Antibody testing on the indicated tissue of (a) clone MIH1 (representative of stain- ing with clones 5-496, 2-272, 16E11, 9A6, 16A4, 6H3, 1105, 25C8E8.F8, and 24B10.G6.D7), (b) clone 5H1, (c) clone 27A2, (d) ETM-80 (stained similarly to ETM-79), and (e) #4059 is shown. The inserted black bars indicate 50 lm.

Article Snippet: All antibodies tested, except the rabbit polyclonal anti-PD-L1 antibody from ProSci (catalogue no. 4059; Poway, CA), either did not stain paraffin-embed- ded tissues (Fig. 1a), gave a high background staining (Fig. 1b,c), or were not blocked by PD-L1 fusion protein competition (Fig. 1d).

Techniques: Immunohistochemistry, Staining, Clone Assay

Journal: Cancer

Article Title: Overall survival and PD-L1 expression in metastasized malignant melanoma.

doi: 10.1002/cncr.25747

Figure Lengend Snippet: Figure 3. PD-L1 expression varied during disease progression. PD-L1 staining was scored for the percentage of positive tumor cells. (benign nevi, n ¼ 10; primary tumor, n ¼ 43; satellite meta, n ¼ 8; in-transit meta, n ¼ 20; lymph node meta, n ¼ 21; distant organ meta, n ¼ 22).

Article Snippet: All antibodies tested, except the rabbit polyclonal anti-PD-L1 antibody from ProSci (catalogue no. 4059; Poway, CA), either did not stain paraffin-embed- ded tissues (Fig. 1a), gave a high background staining (Fig. 1b,c), or were not blocked by PD-L1 fusion protein competition (Fig. 1d).

Techniques: Expressing, Biomarker Discovery, Staining

Journal: Cancer

Article Title: Overall survival and PD-L1 expression in metastasized malignant melanoma.

doi: 10.1002/cncr.25747

Figure Lengend Snippet: Figure 2. Histological staining of paraffin-embedded tissues is shown. Depicted are representative tissue samples that stained ei- ther positive or negative for MART-1, GP-100, PD-L1, MHC-I, TGF-b, CD8, CD4, or FoxP3. The inserted black bars indicate 100 lm.

Article Snippet: All antibodies tested, except the rabbit polyclonal anti-PD-L1 antibody from ProSci (catalogue no. 4059; Poway, CA), either did not stain paraffin-embed- ded tissues (Fig. 1a), gave a high background staining (Fig. 1b,c), or were not blocked by PD-L1 fusion protein competition (Fig. 1d).

Techniques: Staining

Journal: Cancer

Article Title: Overall survival and PD-L1 expression in metastasized malignant melanoma.

doi: 10.1002/cncr.25747

Figure Lengend Snippet: Figure 4. Patient overall survival did not correlate with PD-L1 expression. Patients were grouped based on the presence (¼ ever, >1% of tumor cells staining PD-L1 positive) or the ab- sence (¼ never) of PD-L1 expression at any point during dis- ease progression. Numbers at the bottom of the graph represent remaining patients.

Article Snippet: All antibodies tested, except the rabbit polyclonal anti-PD-L1 antibody from ProSci (catalogue no. 4059; Poway, CA), either did not stain paraffin-embed- ded tissues (Fig. 1a), gave a high background staining (Fig. 1b,c), or were not blocked by PD-L1 fusion protein competition (Fig. 1d).

Techniques: Expressing, Staining